As the clocks change here in the UK, we highlight a circadian clock protein KaiB - it utilises a fold-based switch, regulating the cyanobacterial day-night cycle. You can superpose the predicted #AlphaFold model and compare conformers at #PDBeKB:
https://t.co/xTBOwx6VRA
Basics of ElectroSpray Ionization-MS: A delight!
https://t.co/LGOMBcl4Nx
You've been training for this @asmsnews tweeps! Today is THE day to pull out all the stops and V.O.T.E for #TeamMassSpec in the JBC Methods Madness Final Round (aka the Championship)! Go here to vote: https://t.co/nCgdb8XX3M
On #WorldTBDay, here's a reminder of our featured article on the enzyme dihydrofolate reductase (DHFR), a potential target for TB drugs. Our #PDBart calendar image for April 2017 shows the structure of DHFR in pop art style.
🔗https://t.co/9ys61v2fNr
RNA secondary structure visualisation on PDBe pages now displays base pairing information using Leontis-Westhof nomenclature, fully interactive with the 3D @molstar0 viewer. A collaboration with @AntonIPetrov, @GeorgiaTech & @bgsu.
https://t.co/hbRkExN5R9
The wwPDB consortium pays tribute to Dr. Olga Kennard OBE FRS upon the sad news of her passing. Her pioneering work on the development of crystallographic databases laid the groundwork for modern molecular structure data archiving.
Read more: https://t.co/AyiQgv0h1d
Our process for generating data on small molecule ligands and their interactions in the PDB archive has been updated, allowing multi-component ‘bound ligands’ to be identified and defined throughout the PDB archive.
Find out more: https://t.co/ltHiBL5oGw
It's T-5hours @asmsnews tweeps! If you have not yet cast your vote for #TeamMassSpec, giddy-up! Vote here https://t.co/nCgdb8YuTk
#TeamMassSpec and friends, we've made it to the Semi-Finals (Free Radical Four) of the @jbiolchem
#MethodsMadness tournament. Please go vote and RT! One step closer to the 3-peat. Voting closes tonight!
https://t.co/yf9YjVg1VE
Did you know you can download all the structures for a specific protein with one click of a button! This video shows the batch download options on our #PDBeKB aggregated views of proteins. Try it now at https://t.co/1U29B4byh0
To celebrate #StPatricksDay2023, here's a structure of PAT1 from Baker's yeast (5lmg). The protein activates decapping of mRNA and is involved in translational repression. View all the structures at #PDBeKB:
🔗https://t.co/QzdZYtwznT
There are 177 new entries in the PDB this week, including 8 structures of mutants of CODH-II from C. hydrogenoformans, adding to the 16 structures already in the PDB.
View all the structures, plus their ligands and interactions, at #PDBeKB: https://t.co/HTTa1NIvCT
Another great paper from our group - doing HDX-MS, this time, on Spike protein:
Structural dynamics in the evolution of SARS-CoV-2 spike glycoprotein
Nature Communications - In this paper, the authors use hydrogen-deuterium exchange mass spectrometry to describe how t...
rdcu.be
Save the Date for IC-HDX 2024 https://t.co/WyyNQEafF3
#HDXMS
Our #PDBart project brings molecular structures into the art class! This @SciInSchool article by @deeptigupta19 and @DaveASci guides you through the process - a great resource for teachers and those doing scientific engagement.
🔗https://t.co/5Oq5rwQNs9
Step inside a science-inspired art exhibition where students bring biological molecules to life. Now is also a virtual exhibition!
https://t.co/u2FIUJLyKS
We have short tutorials on our YouTube channel, guiding users through using our resources. Click below to find out how you can use #PDBeKB pages to find all ligand interactions for your protein of interest in less than 5 minutes!
https://t.co/sOmSwUa9iX
A reminder of our featured article for March, focusing on DNA structure, which inspired the creation of this wonderful artwork by Ruby from @TheLeys (@Leys_Art).
Read more at https://t.co/qyV8muJiID
New article from the group: proteasome proteoform profiling and quantification using a combination of Top-Down and Bottom-Up proteomics:
Congrats to @angelectron1 ! @IpbsToulouse @ToulouseMass
Establishing 20S Proteasome Genetic, Translational and Post-Translational Status from Precious Biological and Patient Samples with Top-Down MS
The mammalian 20S catalytic core of the proteasome is made of 14 different subunits (α1-7 and β1-7) but ...
www.mdpi.com
Don't forget to check this out @asmsnews twitterati. We know you are a creative bunch. If you're down with 3D printing, show us your stuff! https://t.co/0rkTMrinYY
Calling all @asmsnews 3D print mavens! We want your snappy 3D designs for a special exhibit at #ASMS2023. Learn more and submit your design file for consideration by MARCH 31: https://t.co/0rkTMrinYY
Time to start voting @asmsnews tweeps! This year voting is on the @ASBMB JBC website. To avoid irregular bot activity we presume.
Round 1 closes Fri March 10, VOTE NOW and help us achieve glory once more #TeamMassSpec!
Vote here: https://t.co/PnyI4xitPy
Voting begins today for #JBCMethodsMadness! Pick your favorite methods in the Scientific Sixteen by 12 p.m. West Coast time on Friday (3/10): https://t.co/mfVaISfynV.
AMS 2023 conference will be held in Austin at the University of Texas from July 23-26, 2023. Mark your calendars! For additional details, including session topics and confirmed speakers, visit
Advancing Mass Spectrometry
Advancing Mass Spectrometry for Biophysics and Structural Biology
advancingms.org
Past Lab News: 2022
July 2022. Our simple, two-part protocol for preparation of a maximally deuterated control sample for HDX MS is published in Analytical Chemistry. Read here.
June 2022. A study of the very long-chain acyl-CoA dehydrogenase (VLCAD), a collaboration with Loren Walensky, is published in Nature Communications. Read here
March 2022. Two papers from our exciting array of HDX MS collaborations appear in the Journal of Molecular Biology. Read them here and here
Past Lab News: 2021
December 2021. Our HDX MS data are part of a study led by our collaborator Tom Rapoport explaining how the Cdc48/p97 unfolding machine deals with substrates. Read more.
October 2021. Our collaborative study with Elena Klimtchuk, Lawreen Connors and Olga Gursky at BUSM Amyloidosis Center elucidates how mutations in antibody light chains contribute to the formation of amyloids. Read more.
September 2021. We contribute to a new comprehensive review driven by Mike Guttman’s lab at University of Washington about modern HDX-MS. See the article in Chemical Reviews.
August 2021. We are part of an exciting story about how selected features of the apoptosis protein BAX add stabilization. Read more.
April 2021. Our research is recognized by Northeastern University with an Excellence in Research and Creativity Award. Read more.
Past Lab News: 2020
November 2020. Together with Amy Andreotti’s lab at Iowa State University, we complete a comprehensive study of Bruton’s tyrosine kinase inhibitors. Read more.
October 2020. See our new review of HDX MS, prepared for the Fundamental and Applied Reviews in Analytical Chemistry 2021 issue of Analytical Chemistry. High res version of HDXMS word cloud Figure 1: as a .PNG
June 2020. In work with Loren Walensky at the Dana Farber Cancer Institute, we used HDX MS to investigate the oligomer of BAX, a functional form necessary for mitochondrial apoptosis.
May 2020. Read our review of how hydrogen / deuterium exchange mass spectrometry (HDX MS) and cryo-electron microscopy complement one another in the structural analysis of proteins.
January 2020. A collaboration with Olga Gursky and John Straub from Boston University probed Serum Amyloid A and how it binds to lipids. Read the article here
Past Lab News: 2019-2017
September 2019. Together with Amy Andreotti’s lab at Iowa State University, we show how the PH domain in Tec-family kinases keeps the kinase turned off. Read more.
June 2019. In a collaboration with Tom Rapoport’s lab at Harvard Medical School, HX MS was used to probe substrate unfolding by the Cdc48 ATPase. Full article here.
June 2019. We review HDX MS of chaperones in protein folding. Goes well with previous papers on protein complexes and signaling complexes.
May 2019. Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments.
July 2018. We describe how mutations in the main protein of HDL, apolipoprotein A-I, affect protein conformation. This study is part of a collaboration with the laboratory of Olga Gursky at BUMC. Read article here
April 2018. Our paper about how to study protein complexes by HX MS is published. Read the full article here.
March 2018. Isaac Kresse awarded a Barry Goldwater Scholarship. Read more about Isaac and the other Northeastern University winners.
December 2017. Our collaborative study with Tom Smithgall on the HIV accessory protein Nef is published in the Journal of Molecular Biology. Nef conformation changes during partner binding.
