Hydrogen Deuterium Exchange (HDX)
Mass Spectrometry (MS)
Research Overview
Research in the Engen Laboratory centers around hydrogen deuterium exchange (HDX) mass spectrometry (MS). The technique is described in more detail within the links of this section.
We not only develop new, better and easier ways of doing HDX MS, but we apply these tools to a vast number of different types of proteins. Some of our projects are listed below.
We have many collaborations wherein we primarily apply HDX MS to the study of proteins related to disease. For more details, see also the section on publications.
Tutorial
BACKGROUND / THEORY
• What is it?
• How does it work?
• What is it used for?
• Exchange theory
METHODS
• Labeling methods
• Ionization methods
• Basic experiment
• Controls & calculations
Projects
• Src-family & Tec-family kinases
• Viral accessory proteins
• Apoptosis proteins
• AAA+ family ATPases
• Membrane proteins
• Allostery and protein conformation
• Biopharmaceuticals
• HDX MS methods development
• UPLC at low temperatures
• HX-Express software
• Acid proteases
• Ion-mobility to raise peak capacity
• Digestion under pressure
