Hydrogen Deuterium Exchange (HDX)
Mass Spectrometry (MS)

Hydrogen Deuterium Exchange (HDX)
Mass Spectrometry (MS)

Research Overview

Research in the Engen Laboratory centers around hydrogen deuterium exchange (HDX) mass spectrometry (MS). The technique is described in more detail within the links of this section.

We not only develop new, better and easier ways of doing HDX MS, but we apply these tools to a vast number of different types of proteins. Some of our projects are listed below.

We have many collaborations wherein we primarily apply HDX MS to the study of proteins related to disease. For more details, see also the section on publications.


• What is it?
• How does it work?
• What is it used for?
• Exchange theory

• Labeling methods
• Ionization methods
• Basic experiment
• Controls & calculations


• Src-family & Tec-family kinases
• Viral accessory proteins
• Apoptosis proteins
• AAA+ family ATPases
• Membrane proteins
• Allostery and protein conformation
• Biopharmaceuticals

• HDX MS methods development
• UPLC at low temperatures
HX-Express software
• Acid proteases
• Ion-mobility to raise peak capacity
• Digestion under pressure